Wednesday 27 January 2016

Year 7 Examine DNA

Not quite Silent Witness but we have extracted the DNA from an onion during our work on reproduction.
First get your equipment ready 
and read the instructions 
 
mix the onions with salt water
 
and grind together to crush the cells and dissolve the DNA 
the more its ground the better 
 
Then we need to filter the insoluble onion out of the mixture
We use cloth to filter the insoluble bits
The residue stays in the filter funnel 
The filtrate passes through into the measuring cylinder
 
We have to collect 50ml
 
 
 
 
 
Then we add washing up liquid to the filtrate 
this must be stirred in carefully as we don't want any froth
One class recorded their experiment photographically using the ipad 
Careful mixing
 
Just one spoonful
 
 
 
Now we need cold ethanol
This must be poured in carefully 
down the edge of the beaker
 
so that the ethanol floats on top of the solution
 if you are careful enough
then you get two distinct layers 
with a sort of white fluffy boundary 
this white stuff is the DNA 
which looks stringy 
well there is about 1m length of it in each cell! 
The DNA is the middle layer here