They took 20 ml of starch solution
And had a spotting tile ready with drops of iodine in each well
Each group added a different amount of amylase enzyme
The enzyme will chemically digest the long chain starch molecules
Cutting them into smaller glucose molecules
When the starch is tested with iodine it turns black
The enzyme is added to the starch
The mixture is agitated with the pipette
The timer is started when the solutions are mixed
Then every 30 seconds a drop of the mix is added to a new well on the spotting tile
If it goes black there is starch left and the enzyme has not digested it all
But when it does not turn black it means the enzyme has chopped up all of the starch and it is turning into glucose.
We keep going until the iodine stays brown
These are the results
The more amylase that was added the quicker the starch was digested
Small amounts of amylase (5ml) took the longest
And large (15 ml) took much less time
#Each well in the tile corresponds to 30s in time
It was a very successful experiment.
